Introducing the ZOE™ Fluorescent Cell Imager.

With an intuitive touch-screen interface and a large field of view, you can view your cells, capture high-resolutions images and perform multichannel merges with ease.

Cell imaging simplified.

No dark room, no training, no overwhelming user interface.

With thoughtful features like an integrated light shield and an intuitive touch-screen, ZOE™ provides the flexibility to carry out fluorescent cell imaging at your bench, right where you work. Combining bright-field capabilities with multi-channel fluorescence, this cell imager is both affordable and easy to use – your perfect solution for routine cell culture and imaging applications.

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    Take the ZOE™ lmager for a Test Drive

    Both you and your students can take ZOE™ for a test drive. Just launch the ZOE™ Interactive Web App to try the intuitive ZOE™ user interface, manipulate images, view features and specifications in depth, and more.

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    Testimonials

    "With the touch screen and how you zoom in and out, this type of technology, I believe, is going to be the preference of the younger generation."

    "For a traditional microscope you have the scope, the camera, the computer. It takes up a lot of space. With ZOE™, the fact that everything is in one machine that’s portable — I really liked it,"
    Dr Checo Rorie Assistant Professor of Genetics, North Carolina A&T State University University of Cambridge
    "It’s very iPad-like — you just turn it on and the icons on the screen are self-explanatory."

    "The fact that ZOE™ has a monitor, that multiple people can look at the same thing at the same time, was what we liked about it; the ability to interact with other people while you are looking at the same thing."
    Dr Nicholas Maness Assistant Professor, Division of Microbiology, Tulane University
    "It really did not need any dedicated bench space and the images we obtained were instantaneous and quite beautiful. Using it is totally intuitive, with a touch screen interface and the ease of a personal tablet—any 18- to 25-year-old student can be taught how to use it in a few minutes. That’s very valuable to us since we have a lot of undergraduates in our lab who need to be taught things very quickly."
    Read more >
    Dr Theodore Price Assistant Professor, School of Behavioural and Brain Science, University of Texas
    "...it is already being used daily! It really is an essential piece of equipment to us which had previously been lacking and dramatically improves the work we do with cells. Iit is designed so that we don’t need a dedicated ‘dark room’ to use it. Much easier as it frees up space in the department and brings the microscope closer to the cells to avoid all sorts of potential problems. Furthermore, the screen on the microscope allows us to train other researchers and allows all of us to look at the cells simultaneously during assessment."
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    Dr Carl Spickett Post-doctoral researcher, Laboratory of Medical Genetics, University of Cambridge
    "The strength of the instrument is certainly the small footprint and the quick access. You don’t really have to turn off the light, you can just monitor. It’s a great confirmation that everything is fine."

    "I could actually look quickly, screen my plates, and see what the expression levels are without even turning down the light. I just put the plate on the instrument — that’s powerful."

    "The easier it is to access [fluorescence imaging] and the easier you make it to do good documentation, the more people will stick to the documentation."
    Dr Michael Menze Assistant Professor, Biological Sciences, Eastern Illinois University
    "To [confirm viral infection] with a fluorescence microscope in the past meant separately exporting each fluorescent and brightfield image and overlaying the images using editing software. ZOE streamlines the process by allowing touchscreen image selection and tapping the merge option to quickly obtain an overlaid image."
    Read more >
    Dr Jody Martin Lab Director, Cell & Molecular Physiology Department, Loyola University Medical Center

    Specifications

    • Imaging channels

      Brightfield and three fluorescence channels*

       

      *High-quality hard-coated filter sets provide high light transmission and long life.

    • Illumination light sources

      Ultraviolet (UV) LED for blue channel
      Blue LED for green channel
      Green LED for red channel
      Ring of green LEDs for brightfield channel (reduces chromatic aberration)

    • Blue channel

      Excitation: 355/40 nm; emission: 433/36 nm

    • Green channel

      Excitation: 480/17 nm; emission: 517/23 nm

    • Red channel

      Excitation: 556/20 nm; emission: 615/61 nm

    • Touch Screen

      25.6 cm (10.1 in.) color LCD monitor, touch screen with antiglare and antifingerprint treatment, 1,280 x 768 pixels image resolution, 80–180° angle tilt range

    • Focusing mechanism

      Coarse and fine, manual adjustment

    • Camera

      Monochrome camera, 12 bit CMOS, 5 megapixels

    • Digital zoom magnification

      700x

    • Motorized stage

      6 mm travel in X, Y direction; touch-screen control of travel speed and direction

    • Compatible consumables

      Flasks (T25, T75, T225); multiwell plates (6, 12, 24, 48, 96, and 384 wells); dishes (35, 60, and 100 mm); chamber slide; glass microscopy slide

    • Imaging file types

      JPEG, TIFF, RAW

    • Image merge

      Images from up to 4 channels can be overlaid

    • Data storage

      16 GB internal memory (~2,500 JPEG files, 1,500 TIFF files, 400–800 RAW files)

    • Data export

      Yes, 2 USB ports

    • Display output

      Yes, through HDMI port

    • Objective

      20x

    • Numerical aperture

      0.40

    • Field of view

      0.70 mm2

    • Display magnification

      175x

    • Onboard software

      Yes

    • Stand-alone system

      Yes, Android operating system; PC is not required for operation

    • Instrument dimensions
      (W x D x H)

      33 x 32 x 30 cm (13 x 12.6 x 11.8 in.)

    • Instrument weight

      9 kg (19.8 lb)

    Download PDF

    Frequently asked questions

    • Can the ZOE™ be used in ambient light?

      Yes, with its integrated light shield fluorescence imaging can be done anywhere in the lab.

    • What applications can I use the ZOE™ for?
      • Estimate cell confluency
      • Observe general cell health and morphology
      • Monitor cell growth and proliferation
      • Capture brightfield or fluorescence images of cells
      • Visualise expression of fluorescent proteins
      • View immunofluorescent localization of proteins
      • Estimate transfection efficiency
    • What fluorophores are compatible with the ZOE™?

      You can download a complete list of compatible fluorophores here.)

    • What vessel types can I use?

      ZOE's™ large open stage is compatible with all cell culture vessel types without any adaptors.

      • Flasks: T25, T75, or T225
      • Multi-well plates: 6-, 12-, 24-, 48-, 96-, or 384-well microplates
      • Dishes: 35 mm, 60 mm, or 100 mm
      • Slides: chamber slides or standard glass microscopy slides
    • How many images can be stored in the ZOE’s™ memory?
      • 2500 JPEGs
      • 1500 TIFFS
      • 400-800 RAW
    • The ZOE™ does not have phase contrast, how can I optimize contrast?

      While ZOE™ does not have phase contrast hardware, it uses green colour LEDs (for reduced chromatic aberration) and oblique illumination to achieve phase like image contrast.

    • How do I optimize imaging parameters using the touch screen?
      • When searching for your sample — increase Gain value for brighter signal; reduce Exposure time for faster frame rate
      • Once the sample is identified and ready to be captured — lower Gain value to reduce background noise; increase Exposure time to regain signal intensity
      • To further increase brightness — start by increasing LED Intensity value; followed by increasing Exposure time if needed
      • To reduce nonspecific background signal — increase Contrast value
    • How do I optimize the brightfield channel
      • Turn off 3 quadrants (tap the checkmarks)Leave only Q2 (or only Q3) on
      • The quadrant that provides the best contrast varies depending on cell culture vessel type and sample
      • Try other quadrants only once the Gain, LED Intensity and Contrast values have been optimized (turn off Q3 and turn on Q4 etc.)
      • Increase Gain and LED Intensity to compensate for loss of light
      • Increase Contrast as needed
    • How do I perform a multicolor merge?
      • When capturing images that will be overlaid, all images should come from the same field of view and must be of the same magnification (use the scale bar displayed in the lower right corner during image capture as a guide).
      • In Live mode, turn on the light source you wish to use.
      • Adjust the settings in Edit if desired and tap the Capture icon.
      • Live mode will switch to Gallery mode and the just-captured image will be displayed. If satisfied with the image, tap Select; the selected image will be uploaded in Merge mode. The merge selection indicator will be updated to show all currently selected colors.
      • Return to Live mode and repeat steps 2 and 3 for the remaining colors you wish to use in the merge.
      • Go to Merge mode, in which all selected images are automatically overlaid.
      • Optimize brightness and contrast in Edit if desired, then tap Save to save the merge as a single image file in the merge folder (in Gallery mode), from which it can be exported to a USB flash drive.
    • How do I update the firmware?

      You can download the latest version of the ZOE firmware here.

    • How to optimize imaging parameters (Live mode) using the Edit menu in any imaging channel
      • When searching for sample — increase Gain value for brighter signal; reduce Exposure time for faster frame rate
      • Once the sample is identified and ready to be captured — lower Gain value to reduce background noise; increase Exposure time to regain signal intensity
      • To further increase brightness — start by increasing LED Intensity value; follow by increasing Exposure time if needed
      • To reduce nonspecific background signal — increase Contrast value
    • Brightfield channel: phase like image contrast with oblique illumination
      • Turn off 3 quadrants (tap the checkmarks)
        • Leave only Q2 (or only Q3) on
        • The quadrant that provides the best contrast varies depending on cell culture vessel type and sample
        • Try other quadrants only once the Gain, LED Intensity and Contrast values have been optimized (turn off Q3 and turn on Q4 etc..)
      • Increase Gain and LED Intensity to compensate for loss of light
      • Increase Contrast as needed
    • Firmware Update Procedure
    • How to create a Multicolor Merge
      • When capturing images that will be overlaid, all images should come from the same field of view and must be of the same magnification (use the scale bar displayed in the lower right corner during image capture as a guide).
      • In Live mode turn on the light source you wish to use.
      • Adjust the settings in Edit if desired and tap the Capture icon.
      • Live mode switches to Gallery mode and the just-captured image is displayed. If satisfied with the image, tap Select; the selected image is uploaded in Merge mode. The merge selection indicator is updated to show all currently selected colors.
      • Return to Live mode and repeat steps 2 and 3 for the remaining colors you wish to use in the merge.
      • Go to Merge mode, in which all selected images are automatically overlaid.
      • Optimize brightness and contrast in Edit if desired, then tap Save to save the merge as a single image file in the merge folder (in Gallery mode), from which it can be exported to a USB flash drive.
    • What vessel type are compatible?
      • Flasks: T25, T75, or T225
      • Multiwell plates: 6-, 12-, 24-, 48-, 96-, or 384-well microplates
      • Dishes: 35 mm, 60 mm, or 100 mm
      • Slides: chamber slides or standard glass microscopy slides
    • Does ZOE™ require dark room for imaging?

      Integrated light shield enables fluorescence imaging in ambient light, dark room is not needed.

    • Can I image samples in cell culture flasks?

      Yes, the objective lens is located below the stage, thus making imaging samples in cell culture vessels possible.

    • Can ZOE™ capture images and store the image files?

      Yes, ZOE has integrated digital camera (monochrome 12 bit CMOS, 5 megapixels) that is controlled through touch screen. Captured images are stored in 16GB internal memory.

    • Do I need special software to open these image files?

      The images files (JPEG, TIFF, or RAW) are compatible with commonly used imaging software packages.

    • What are the light sources ZOE™ cell imager uses?

      LEDs are used as light sources for both brightfield and fluorescent channels. Unlike mercury arc lamps, that need to be replaced approximately every 300 hours, LEDs provide thousands of hours of illumination that are instantly ready for use after power on, without any warm up time. The intensity of the light emitted by the LEDs is user adjustable, thus reducing sample photo-bleaching.

    • Can I replace filters or light sources?

      ZOE™ is fully integrated and its fluorescence channels are optimized for commonly used fluorescent proteins and dyes. While not offering the ultimate flexibility of filter/light source selection, a large number of fluorophores is compatible with ZOE™ giving user a large degree of flexibility and choice of fluorophores – allowing them to do three color fluorescence experiments in an easy to use and affordable instrument format.

      • Blue channel Excitation: 355/40 nm; emission: 433/36 nm
      • Green channel Excitation: 480/17 nm; emission: 517/23 nm
      • Red channel Excitation: 556/20 nm; emission: 615/61 nm
    • Sample focusing – using the Z axis indicator

      Focus position indicator (upper left side) shows the Z axis position number; the number is updated during focusing. Knowing the approximate Z number for the tissue culture vessels you use can speed up focusing.

      Vessel TypeZ Axis Position
      Microscope slide720 (± 100)
      6-well slide2,000 (± 100)
      12-well slide2,200 (± 100)
      24-well slide1,900 (± 100)
      48-well slide2,000 (± 100)
      96-well slide3,500 (± 200)
      T25 flask1,800 (± 200)
      T75 slide2,500 (± 200)
      T225 tissue culture flask3,200 (± 200)
      Small petri dish1,200 (± 200)
      Large petri dish100 (± 200)
    • Can the instrument be attached to a computer?

      The system could be damaged if anything in ZOE™ cell imager was deleted (images, folders) through the PC or if anything was copied (images, programs) into the ZOE™ cell imager through the PC.

      Images not exported through the USB port will be monochrome, the pseudo-coloring of images from fluorescent channels occurs when exported through USB port.

    • Can the ZOE™ cell imager be connected to an external monitor?

      Yes, it can be connected through the HDMI port in the back of the instrument. Note: Not all adaptors used for projectors/monitors without HDMI port are compatible with ZOE firmware. If upon connection the cell imager can not exit the loading screen then the adaptor is not compatible.

    • What are the calibration requirements?

      There are no calibration requirements, the ZOE™ cell imager is factory calibrated.